Twelve human semen specimens were to compare the reliability of the eosin, trypan blue, and hoechst supravital tests. The acridine orange test was also carried out to investigate whether the score of this test has a relation to the results of
supravital
testing. All specimens were tested in duplicate. The percentages of live sperm found by eosin, trypan blue, and Hoechst staining were 50-88%, 46 -89%, and 48%-87%, respectively. The differences are not statistically significant. The sperm
motility
scores had no relation to the supravital staining results, as many sperm were viable even though motility was 0.
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